This invention facilitates the high-throughput analysis of gene functions. In this technology, gene expression constructs or siRNAs are arrayed on an electrode, and mammalian cells are cultured on the array. At desired moment, electric pulses are applied to the system to allow for parallel introduction of the nucleic acids into the cells. Specific phenotypes caused by overexpression or silencing of genes provide biological readouts from which we can infer the functions of particular genes. What is unique with our technology is that it performs surface-mediated electroporation, which enables us to transfect cells in highly efficient and temporally controlled manners.

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