Technology Information
This technology is a protein labeling system utilizing a protein tag and its specific ligand. Inventors have developed PYP (Photoactive Yellow Protein; 14kDa) as the candidate tag. Also they designed and synthesized a novel fluorescent probe containing coumarin. When a PYP-fused target protein is labeled with the probe that is nonfluorescent before the labeling reaction, the probe bound to the fusion protein emits strong fluorescence. This property allows selective fluorescence detection of the fusion protein with high sensitivity in vitro or living cells.




Competitive Advantages
1. This system does not require washing procedures to remove the unreacted probes after labeling.
2. It can be used for the specific labeling of proteins in animal cells because PYP is absent in those cells.

Applications
Bio-imaging system
It is useful to visualize the localization and behavior of particular proteins.

Categories
Research Tool / Labeling System / Fluorescent probes / Fluorescence microscope

Further Detail information is available under CDA

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