Background
Regeneration medicine is one of the breakthroughs in recent biomedical industry, and autogenous skin or corneal tissue transplantation is actually conducted in medical practices. One of the main obstacles for industrialization is to clear the safety issue required by the regulatory authorities. In many cases feeder cells should be applied for efficient cell culturing, and currently mouse derived fibroblast such as 3T3NIH is commonly used as feeder cell. However even as feeder cell, use of xenogeneic cell is characterized as xenotransplantation and large amount of requirements are imposed for commercial use. With all enthusiastic studies of human derived feeder cell or feeder-less culture system, no practical universal methods free from xenogeneic cell have been established.

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