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Modulator for expression of human INF-alpha1 (IFN-a1 ) mRNA
We discoverd that natural antisense RNA (AS) is an important modulator of human IFN-α1 mRNA stability, which targets a local single-stranded region within the conserved secondary structure formed by the coding sequence of IFN-α1 mRNA.
Following infection by the Sendai virus (a paramyxovirus) expression of IFN-α1
AS becomes elevated. This elevated expression results in increased IFN-α1 mRNA stability and, subsequently, IFN-α protein production through the cytoplasmic (but not nuclear) interaction of AS with the mRNA at the local single-stranded region. The silencing of IFN-α1 AS by sense oligonucleotides and overexpression of antisense oligoribonucleotides, which were both designed from the local target region, confirmed the critical role of the AS in the posttranscriptional regulation of IFN-α1 mRNA expression. Importantly, this IFN-a1 AS expression was also confirmed with IFN-a1 mRNA in the respiratory pathway of model animals, when they were infected with human Influenza A virus (H1N1O), indicating that AS may become a target to develop antiviral nucleic acid medicines. To access the rest of the information on this page,
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