Field
     Tissue engineering

Keywords
     Gene delivery, Fe3+, Material transduction, Tissue engineering

Advantages
    Incorporation into the cells thereby is more efficient than a DOTAP reagent and, moreover, as a
    result of the means by fusion, a high effect is achieved in the induction of gene, etc.
    Since liposome is able to be efficiently incorporated into the cells, there is less risk in terms of
    safety than the method where phage, plasmid, vector, etc. are used.  The efficiency is higher
    than electroporation.  In addition, although it is a matter of course that the incorporating
    efficiency into the cells of a suspended state is high, the efficiency of incorporation in the cells
    which are subjected to adhesion culture is also very high.  It is now possible to solve the
    conventional problem that efficiency of induction of gene into the cells in an industrial scale is
    poor.

Abstract
There are several methods for the incorporation of gene or protein into the cells but they have various problems and take on difficulties that the final efficiency is low whereby industrialization thereof is not possible.
 The present method is a method where gene, protein or chemical substance which is to be put into the cells is included in liposome and is incorporated into various cell species by means of fusion in the presence of trivalent iron ion.  The incorporating efficiency by the method is higher than that of the conventional apparent efficiency and is as high as more than 40%.  It has also a characteristic feature that no decomposition happens due to the incorporation by means of fusion whereby it plays an important role as an element technique for industrialization of product production, regenerative medicine, etc.

                   
Inventor
     Prof. Makoto Haga　et al.　(Tokyo University of Science)


Patent Publication No
     (APPLICATION NUMBER, FILING DATE)
     WO2010069767（Oct. 29, 2010年）
     (PRIORITY APPLICATION NUMBER, PRIORITY DATE)
     US61/256336 (Oct. 30, 2009 US provisional application)

Internal file number
     LSP:61
     G2010-013 (G2009-008)

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